After winning a gold medal for classical guitar, and then the Diplôme d'État de professeur de guitare, Nicolas Muzy practised the traditional music of the Andes and Argentina, before turning to early music. He entered the Conservatoire National Supérieur de Musique de Lyon, where he studied theorbo, early guitars and lute with Eugène Ferré. He obtained his diploma with honours in 2007. Nicolas Muzy continued his training in courses and academies, performing under the direction of William Christie, Philipp Pickett, Emmanuel Mandrin and Christophe Coin, and studying baroque declamation with Eugène Green and Nicole Rouillé.

In 2010, Nicolas Muzy founded the ensemble Séléné, a quartet dedicated to 17th-century chamber music. The ensemble regularly works with actors and singers on programmes combining music and theatre.

He is currently continuo player and soloist with several early music ensembles in France and Spain: Les Nouveaux Caractères (Sébastien d'Hérin), Le Concert de l'Hostel Dieu (Franck-Emmanuel Comte), Ensemble Alkymia (Marianna Delgadillo), le Tendre Amour (Esteban Mazer), Les Arts Florissants (W. Christie)...

Among his teaching activities, Nicolas Muzy is an instructor at the Centre culturel de rencontre d'Ambronay and teaches the lute at the Conservatoire de Chambéry, where he was also coordinator of the early music department.